A 70% Ethanol Extract of Mistletoe Rich in Betulin, Betulinic Acid, and Oleanolic Acid Potentiated β-Cell Function and Mass and Enhanced Hepatic Insulin Sensitivity

Figure 7

Glucose-stimulated insulin secretion and cell viability in insulinoma Min6 cells. Min6 insulinoma cells were treated with low (0.5 μg/mL) or high (5 μg/mL) doses of vehicle (DMSO) or low (5 μM) or high (50 μM) doses of betulin, betulinic acid, and oleanolic acid for 16 hours. At the end of the incubation, media were changed into high glucose (20 mM) Krebs-Ringer-Hepes buffer containing assigned compound for 30 min and insulin concentrations in the buffer were measured by RIA insulin kit (a). After treatment with vehicle (DMSO) or low (5 μM) or high (50 μM) doses of betulin, betulinic acid, and oleanolic acid for 48 h and glucose-stimulated insulin secretion measured for 30 min, cell viability was measured with a cell proliferation WST-1 reagent assay kit (b). The bars and error bars represent mean ± SD (). Values on the bars with different superscripts were significantly different in a Tukey post hoc test at a significance of .

(a)

(b)