Research Article

Composition Analysis and Inhibitory Effect of Sterculia lychnophora against Biofilm Formation by Streptococcus mutans

Figure 6

Bactericidal effect of S. lychnophora cultured S. mutans treated with S. lychnophora and stained with LIVE/DEAD BacLight Bacterial Viability Kit. Stained bacteria were observed using confocal laser scanning microscopy. Treatment with S. lychnophora decreased green-labeled living bacteria (SYTO 9 stain) and increased red-labeled dead bacteria (PI stain) dose-dependently. (a) Control, (b) 0.04, (c) 0.08, (d) 0.16, and (e) 0.32 mg/mL extract and (f) positive control sodium fluoride 0.1% (NaF), scale bar = 50 μm, objective lens (×100).