The Zuo Jin Wan Formula Induces Mitochondrial Apoptosis of Cisplatin-Resistant Gastric Cancer Cells via Cofilin-1

Figure 3

ZJW induces mitochondrial injury in DDP-resistant SGC7901/DDP cells. (a) ZJW decreased the mitochondrial membrane potential () of SGC7901/DDP cells. SGC7901/DDP cells were treated with 50 μg/mL ZJW for 0, 12, and 24 h. Then was evaluated using flow cytometry with the fluorescent indicator JC-1 as the degree of change in the ratio of red (FLH-2) to green (FLH-1) fluorescence. , versus control groups treated with ZJW for 0 h. (b) A cell fluorescence assay detected the FLH-2 and FLH-1 fluorescence intensities of JC-1 in SGC7901/DDP cells after exposure to ZJW. (c) ZJW induced intracellular calcium overloading in SGC7901/DDP cells. The intracellular calcium was evaluated using flow cytometry with the fluorescent indicator Fluo-3 AM. , versus control groups treated with ZJW for 0 h. (d) A cell fluorescence assay detected the FLH-1 fluorescence intensity of Fluo-3 AM in SGC7901/DDP exposed to ZJW.

(a)

(b)

(c)

(d)