The Zuo Jin Wan Formula Induces Mitochondrial Apoptosis of Cisplatin-Resistant Gastric Cancer Cells via Cofilin-1

Figure 6

Cofilin-1 knockdown decreases the apoptosis induced by ZJW in SGC7901/DDP cells. (a) The effects of siRNAs specific for the cofilin-1 gene on cisplatin (DDP)-resistant SGC7901/DDP cells were determined using quantitative real-time PCR. , versus control groups treated control siRNA. (b) The effects of siRNAs specific for the cofilin-1 gene were determined by western blotting. (c) Flow cytometry demonstrated the dose-dependent effects of ZJW treatment on the apoptosis rate of SGC7901/DDP cells 24 h after siRNA-mediated cofilin-1 knockdown. , versus control groups treated with cofilin-1 siRNA and ZJW (0 μg/mL). (d) The viabilities of SGC7901/DDP cells subjected to cofilin-1 knockdown and exposed to 0, 25, 50, 100, 200, or 400 μg/mL ZJW for 24 h were determined using a CCK-8 assay. (e) SGC7901/DDP cells with cofilin-1 knockdown exposed to 50 μg/mL ZJW for 24 h, depolymerisation of F-actin, and translocation of G-actin and cofilin-1 from the cytoplasm to the mitochondria were detected using immunofluorescence assay.

(a)

(b)

(c)

(d)

(e)