The Effect of Repeated Electroacupuncture Analgesia on Neurotrophic and Cytokine Factors in Neuropathic Pain Rats

Figure 3

Effect of EA treatment on spinal BDNF expression on astrocyte in different groups. The expression of BDNF is shown in red and that of GFAP in green; the coexpression of BDNF and GFAP is yellow. The histogram shows the cell number of the coexpression of BDNF and GFAP (yellow). The yellow area represents the expression of BDNF on the astrocytes. The coexpression level of BDNF and GFAP (yellow) was increased after CCI and decreased after 2 weeks’ EA treatment (EA2W). Pictures 1, 2, and 3 represent magnifications of the merged image in the normal group, the CCI group, and EA2W group, respectively. Picture 4 shows the merged image in the spinal anterior horn after CCI. Scale bar: 200 μm; compared with the normal group; compared with the CCI group.