An Extract of Chinpi, the Dried Peel of the Citrus Fruit Unshiu, Enhances Axonal Remyelination via Promoting the Proliferation of Oligodendrocyte Progenitor Cells

Figure 2

(a) Toluidine blue staining of cross sections of the corpus callosum. Myelin staining was increased in Chinpi-treated mice. Scale bar = 85 μm. (b) Electron micrographs of cross section of the corpus callosum show aging-induced demyelination in control mice. The myelination status was improved after two months of Chinpi treatment. Scale bar = 2 μm. (c) The density of myelinated fibers per 300 μm². Treatment with Chinpi led to a significant increase in the myelinated nerve fiber density. The number of myelinated fibers was counted using five electron micrograph images from each mouse. Data represent mean ± SEM (): . (d) The -ratio, an indicator of demyelination, which is defined as the ratio of the axon diameter to that of the axon diameter plus the surrounding myelin sheathe (see right panel for formula) was calculated. The increased -ratio in the elderly mice was improved by Chinpi treatment. The number of measured axons was 90, using eight electron micrographs from three mice. Data represent mean ± SEM. versus control. The analyses were performed using 30-month-old mice.

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