The Function of Naringin in Inducing Secretion of Osteoprotegerin and Inhibiting Formation of Osteoclasts

Figure 2

Effect of naringin on the number of TRAP+ OC cells in calvarial bone cultures. (a) Macroscopic view of TRAP staining in calvarial bone cultures in different experimental conditions (0, 1, 10, and 100 mg/L naringin), after 1, 3, 7, and 10 days (D) in vitro. (b, c, d, and e) Microscopic view of TRAP staining in calvarial bone cultures in different experimental conditions (0, 1, 10, and 100 mg/L naringin), after 10 days (D) in vitro. (f) Quantification of the number of TRAP+ OC cells in bone cultures exposed to 0, 1, 10, and 100 mg/L naringin for 1, 3, 7, and 10 days in vitro. Note the significant reduction in TRAP+ cells at 7 days () and 10 days ().

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