The Function of Naringin in Inducing Secretion of Osteoprotegerin and Inhibiting Formation of Osteoclasts

Table 1

Primer sequences for RT-PCR.

Gene	Primer sequence (forward/reverse)	(annealing)	Cycles
OPG	5′-TCCTGGCACCTACCTAAAACAGCA-3′; 5′-CTACACTCTCGGCATTCACTTTGG-3′	57	35
RANKL	5′-ATGATGGAAGGCTCATGGTTG-3′; 5′-TGTTGGCGTACAGGTAATAGAA-3′	60	35
M-CSF	5′-AAGCAGTAACTGAGCAACGGG-3′; 5′-CCCATATTGCGACACCGAA-3′	59	30
IL-6	5′-GAAATGAGAAAAGAGTTGTGC-3′; 5′-ATTGGAAATTGGGGTAGGAAG-3′	56	35
-actin	5′-TGGAATCCTGTGGCATCCATGAAAC-3′; 5′-TAAAACGCAGCTCAGTAACAGTCCG-3′	61	30