New Claims for Wild Carrot (Daucus carota subsp. carota) Essential Oil

Figure 4

Anti-inflammatory effect of Daucus carota subsp. carota in LPS-stimulated Raw 264.7 macrophages: (a) NO production and (b) cell viability. Macrophages were treated with essential oil (1.25–0.08 μL/mL) for 1 h prior to LPS (1 μg/mL) activation and further incubated for 24 h. NO release was determined in the supernatants of the cultures using the Griess reagent (a) and cell viability was assessed on adherent cells using the resazurin reagent and expressed as percentage of cell viability by control cells (b). Results are shown as mean ± SEM of at least three independent assays. (; ; , compared to LPS). Cell viability control (100%) corresponds to an absorbance mean value of 0.435.

(a)

(b)