Research Article

Asteraceae Artemisia campestris and Artemisia herba-alba Essential Oils Trigger Apoptosis and Cell Cycle Arrest in Leishmania infantum Promastigotes

Figure 3

Morphological effect of the essential oils on L. infantum promastigotes. The figure illustrates the Giemsa staining of L. infantum promastigotes with no treatment (a) or after treatment with 0.5 μL/mL EO of A. herba-alba (b), 0.5 μL/mL EO of A. campestris (c), or 0.5 μg/mL amphotericin B (d). The slides were observed at 40x and 100x magnifications. Untreated cells show ordinary elongated cell bodies while those stimulated with EO present remarkable shrinkage of the body. Treatment with amphotericin B results in swollen promastigote bodies. The arrows point to shrunken promastigote bodies while the circles highlight the swollen promastigote bodies.
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