Evidence-Based Complementary and Alternative Medicine

Research Article

Identification of the Metabolic Enzyme Involved Morusin Metabolism and Characterization of Its Metabolites by Ultraperformance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS/MS)

Table 1

of [M-H]− measurements for the protonated molecules of metabolites in rat, monkey, pig, rabbit, human, and dog liver microsomes.
NumberFormulaMassFragmentError
(ppm)		Metabolic reactionSpecies
RatsMonkeyPigsRabbitsHumanDog
M09.17C25H24O6419.1545350, 349, 335, 321, 309, 297, 217, 201, 191, 147, 121+1.1ParentDDDDDD
M18.66C25H24O7435.1392377, 325, 313, 217, 191, 121+0.2HydroxylationDDDDDD
M27.15C25H24O7435.1451417, 393, 377, 375, 351, 313, 217, 191−0.2HydroxylationDDDDDD
M37.50C25H22O7433.1195401, 379, 361, 346, 291−0.1Hydroxylation + dehydrogenationDDDDDND
M47.09C25H22O7433.1285401, 361+2.2Hydroxylation + dehydrogenationDDDNDNDD
M58.82C25H26O8453.1515435, 350, 332, 325, 313, 309, 285, 235, 217, 191, 121+0.62 × hydroxylation + reductionDDDDDD
M68.10C25H26O8453.1528421, 393, 381, 350, 325, 313, 285, 235, 217, 191, 121−2.62 × hydroxylation + reductionDDDNDDD
M78.34C25H26O7437.1389419, 365, 339, 315, 297, 257, 219, 217, 191, 121+1.9HydrationDDDDDD
M88.77C25H24O8451.1393433, 419, 381, 375, 360, 333+0.22 × hydroxylationDDDDNDND
D, detected; ND, not detected.