Evidence-Based Complementary and Alternative Medicine / 2016 / Article / Fig 4

Research Article

Inhibitory Effect of Valencene on the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Figure 4

Effects of valencene on the expression/production of chemokines and intercellular adhesion molecule in HaCaT cells. HaCaT keratinocytes were pretreated with valencene (10, 50, or 100 μM; 1 h) and then stimulated with TNF-α (10 ng/mL)/IFN-γ (10 ng/mL) for 24 h. (a) CCL17/TARC, CCL22/MDC, CXCL8, MCP-1, GM-CSF, and I-CAM levels were determined in culture supernatants using commercial detection kits and (b) corresponding mRNA expression was determined by RT-PCR. (c) Effects of valencene on cell viability was assessed by XTT. HaCaT cells were treated with VAL for 24 h at the indicated concentration. Data were expressed as the means ± SDs ( per experiment). versus TNF-α/IFN-γ alone.

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