Electroacupuncture Attenuates Cerebral Ischemia and Reperfusion Injury in Middle Cerebral Artery Occlusion of Rat via Modulation of Apoptosis, Inflammation, Oxidative Stress, and Excitotoxicity

Table 1

All the primers information used in quantitative real-time PCR.

Gene		Primer sequence
GCSh	Forward primer	5′-TATCTGCCCAATTGTTATGGCTTT-3′
	Reverse primer	5′-TCCTCCCGTGTTCTATCATCTACA-3′
	Probe	5′-CATCGCCATTTTACCGAGGCTACGTG-3′
GCSl	Forward primer	5′-GGGCACAGGTAAAACCCAATAG-3′
	Reverse primer	5′-TTGGGTCATTGTGAGTCAGTAGCT-3′
	Probe	5′-TTAATCTTGCCTCCTGCTGTGTGATGCC-3′
Nrf2	Forward primer	5′-CCATTCCCGAGTTACAGTGTCTT-3′
	Reverse primer	5′-GATCGATGAGTAAAAATGGTAATTGC-3′
	Probe	5′-CAGCCCAGAGGCCACACTGACAGA-3′
IL-1β	Forward primer	5′-TGTGATGAAAGACGGCACAC-3′
	Reverse primer	5′-CTTCTTCTTTGGGTATTGTTTGG-3′
	Probe	5′-AGCTGGAG-3′
TNF-α	Forward primer	5′-TGAACTTCGGGGTGATCG-3′
	Reverse primer	5′-GGGCTTGTCACTCGAGTTTT-3′
	Probe	5′-AGGAGGAG-3′
IL-6	Forward primer	5′-CCCTTCAGGAACAGCTATGAA-3′
	Reverse primer	5′-ACAACATCAGTCCCAAGAAGG-3′
	Probe	5′-CCAGCCAG-3′
GAPDH	Forward primer	5′-CCTCAAGATTGTCAGCAATGCA-3′
	Reverse primer	5′-TGGCAGTGATGGCATGGA-3′
	Probe	5′-CACCACCAACTGCTTAGCCCCCCT-3′