Flavonoids and Sesquiterpene Lactones from Artemisia absinthium and Tanacetum parthenium against Schistosoma mansoni Worms

Figure 2

Confocal laser scanning microscopy observations of S. mansoni male worms after in vitro incubation with parthenolide. Adult worms were incubated in 24-well culture plates containing RPMI 1640 medium with 0.5% DMSO and treated with parthenolide at different concentrations. (a) General view of the anterior worm region showing, in red, the location where tegument was analyzed. (b) Control containing RPMI 1640 with 0.5% DMSO, showing tubercles (T). (c) 12.5 μM parthenolide, showing tubercles shrunken (sh) and disintegrated (di). (d) 25 μM parthenolide, showing surface sloughing (sl). (e) 50 μM parthenolide, showing tubercles disintegrated (di). (b, d) Bars = 200 μm. (c, e) Bars = 50 μm.

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