Vascular Protective Role of Samul-Tang in HUVECs: Involvement of Nrf2/HO-1 and NO

Figure 5

(a) Effects of SMT on TNF-α induced phosphorylation of IκB-α in HUVECs. (b, c) Effects of SMT on TNF-α induced NF-κB translocation in HUVECs. Cells were treated with TNF-α (50 ng/mL) for 1 hour in the absence or pretreatment of SMT (10, 30, and 50 μg/mL) for 30 min. NF-κB protein was detected by western blot and immunofluorescence. (Green: NF-κB, blue: nucleus; magnification: 400x.) Bar represents the mean ± SEM of 3 independent experiments. and versus con group. and versus TNF-α group.

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