Vascular Protective Role of Samul-Tang in HUVECs: Involvement of Nrf2/HO-1 and NO

Figure 6

Effect of SMT on TNF-α induced NF-κB activation in HUVECs. Cells were treated with TNF-α (50 ng/mL) for 1 hour in the absence or pretreatment of SMT (10, 30, and 50 μg/mL) for 30 min and nuclear extracts were prepared to perform (a) electrophoretic mobility shift assay (EMSA) and (b) luciferase promoter assay. Bar represents the mean ± SEM of 3 independent experiments. versus con group. versus TNF-α group.

(a)

(b)