Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3K/Akt/mTOR Signaling Pathway

Figure 4

Promotion of ISO-induced PI3K/Akt pathway activation by JUA. Cells were pretreated with various concentrations (5, 10, and 20 μM) of JUA and exposed to 100 μM of ISO for 6 h; proteins samples were prepared at the indicated time points and analyzed by western blotting. (a) The differences of signaling protein level including phospho- and total-PI3K, AKT, and mTOR proteins expression detected with western blotting analysis. ((b), (c), and (d)) Ratio between phosphorylated and total protein levels was calculated and relative protein quantification in treated versus control extracts was calculated by densitometric analysis. Beta-actin was probed as a loading control. Data represent the mean ± SEM of three independent experiments, and differences between mean values were assessed by one-way ANOVA. versus control group; versus group treated with ISO only; versus group treated with ISO only.

(a)

(b)

(c)

(d)