Identification of Centella asiatica’s Effective Ingredients for Inducing the Neuronal Differentiation

Figure 1

The fractions show wide range of neuronal inductive effects. After the PC12 cells were treated with NGF (50 ng/mL) and fractions (at the same dilution ratio of 1 : 50) for 72 h, randomly selected fields were observed using a camera attached to a microscope (×20). Control (A) 50-3, 50-6, and 75-3; (B) 50-2, 50-5, and 50-10; (C) 50-8, 50-9, 75-1, 75-2, 75-4, 75-5, and NGF. Bar = 50 μm.