Identification of Centella asiatica’s Effective Ingredients for Inducing the Neuronal Differentiation

Figure 4

HPLC-UV and HPLC- analyses of 75-4 (a) and 50-6 (b) under optimized conditions (LC conditions: inject 20 μL; column YMC C18 (250 mm × 4.6 mm, 5 μm); column temperature 25°C; mobile phase: acetonitrile and water gradient the same as those in Table 1, flow rate of 1.0 mL/min; UV: 205 nm; LC- conditions the same as the previous ones, ionization: ESI neg; capillary temperature: 350°C; sheath gas: 40 (arbitrary units); auxiliary gas: 9 (arbitrary units); electrospray voltage: 4 kV; capillary voltage: −45 V; tube lens: −95 V).

(a)  

(b)