Identification of Centella asiatica’s Effective Ingredients for Inducing the Neuronal Differentiation

Figure 5

The combination of asiatic acid and madecassic acid found in 75-4 induces the expression of neurofilament of cultured PC12 cells. (a) Cultured PC12 cells were treated with asiatic acid (14.4 μM), madecassic acid (40.8 μM), their combination, and 75-4 (0.216 mg/mL) for 72 h. The cell lysates were collected to determine the expression of NF68 and NF200. NGF (50 ng/mL) served as the positive control. GAPDH served as loading control. (b) Quantification plot is shown in histograms. Data are expressed as the fold of change (100% of Basal) against the control, mean ± SEM, , pooled from four independent experiments. Statistical comparison was made between asiatic acid, the combination, and 75-4; ; ; .

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