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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 9849134, 10 pages
http://dx.doi.org/10.1155/2016/9849134
Research Article

Structural Aspects of Antioxidant and Genotoxic Activities of Two Flavonoids Obtained from Ethanolic Extract of Combretum leprosum

1Department of Basic Health Sciences, Laboratory of Genetic Toxicology, Federal University of Health Sciences of Porto Alegre (UFCSPA), Rua Sarmento Leite 245, 90150-170 Porto Alegre, RS, Brazil
2National Institute for Translational Research on Health and Environment in the Amazon Region, CNPq/INCT/INPeTAm, Rio de Janeiro, RJ, Brazil
3Department of Pharmacosciences, Laboratory of Genetic Toxicology, UFCSPA, Rua Sarmento Leite 245, 90150-170 Porto Alegre, RS, Brazil
4Núcleo de Ciência e Tecnologia, Universidade Federal de Rondônia, Avenida Presidente Dutra, Até 2965, lado Ímpar, Centro, 76801059 Porto Velho, RO, Brazil

Received 15 March 2016; Accepted 27 April 2016

Academic Editor: Jesus R. R. Amado

Copyright © 2016 Cassiana Macagnan Viau et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Combretum leprosum Mart., a member of the Combretaceae family, is a traditionally used Brazilian medicinal plant, although no evidence in the literature substantiates its antioxidant action and the safety of its use. We evaluated the antioxidant properties of the ethanolic extract (EE) from flowers of C. leprosum and its isolated products 5,3′-dihydroxy-3,7,4′-trimethoxyflavone (FCL2) and 5,3′,4′-trihydroxy-3,7-dimethoxyflavone (FCL5) in Saccharomyces cerevisiae strains proficient and deficient in antioxidant defenses. Their mutagenic activity was also assayed in S. cerevisiae, whereas cytotoxic and genotoxic properties were evaluated by MTT and Comet Assays, respectively, in V79 cells. We show that the EE, FCL2, and FCL5 have a significant protective effect against H2O2. FCL2 showed a better antioxidant action, which can be related to the activation of the 3′-OH in the presence of a methoxyl group at 4′ position in the B-ring of the molecule, while flavonoids did not induce mutagenesis in yeast, and the EE was mutagenic at high concentrations. The toxicity of these compounds in V79 cells increases from FCL2 = FCL5 < EE; although not cytotoxic, FCL5 induced an increase in DNA damage. The antioxidant effect, along with the lower toxicity and the absence of genotoxicity, suggests that FCL2 could be suitable for pharmacological use.