Evidence-Based Complementary and Alternative Medicine / 2017 / Article / Fig 2

Research Article

Maoto, a Traditional Japanese Herbal Medicine, Inhibits Uncoating of Influenza Virus

Figure 2

Maoto inhibited influenza virus propagation in vitro. All cell lines infected with influenza viruses (moi = 1) were cultured for 24 hr; then culture supernatants were assayed for the titer of infectious virus. (a) A549 cells infected with PR8 were cultured with various concentrations of maoto, laninamivir, or amantadine. (b) Left: A549 cells infected with PR8 were cultured with the components of maoto, Ephedra Herb (130 µg/ml), Apricot Kernel (130 µg/ml), Cinnamon Bark (100 µg/ml), or Glycyrrhiza Root (40 µg/ml), according to their weight ratios in maoto (Table 1). Right: A549 cells treated with the control, daikenchuto, another Kampo medicine, were infected with PR8 (moi = 1). (c) A549 cells infected with A/California/7/2009 (H1N1, pdm09), A/Victoria/210/2009 (H3N2), or B/Brisbane/60/2008 were cultured with or without maoto. (d) MEF, HeLa, or HaCat cell lines infected with PR8 were cultured with or without maoto. versus medium only.
(a)
(b)
(c)
(d)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.