Review Article

Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products

Table 5

Different DNA extraction methods that have been used in HMPs.

Method usedExtraction methodReference/supplierMedicinal parts/type of HMPsReferences

Roots, leaves, powder, stem, seeds, whole plantVassou et al. [47]
Leaves and fruitSrirama et al. [119]
JuiceMahadani and Ghosh [120]
Leavesda Costa et al. [121]
Fruits, stem, seeds, herb, cortex, flowers, rhizomeHan et al. [27]
Modified CTAB methodsSalting out precipitationDoyle [122]HerbKumar et al. [123]
LeavesAlexander [124]
HerbLi et al. [125]
LeavesCosta et al. [126]
Dry leaves, bark, fruitSeethapathy et al. [127]
LeavesSelvaraj et al. [128]
PowderSheth and Thaker [129]
HerbsCheng et al. [130]
RootsZhou et al. [131]
RhizomeWong et al. [132]

Modified SDS methodsSalting out precipitationLeaves, flower, budTamari et al. [133]
Edwards et al. [134] LeavesAlexander [124]
Leavesda Costa et al. [121]
HerbsCheng et al. (2014)

Wizard Genomic DNA purification kitSilica bindingPromegaTeaJian et al. [135]

Capsule, tablets, powder, leafNewmaster et al. [20]
Dried and fresh plant tissue, capsule, tea, dried barkSingtonat and Osathanunkul [136]
NucleoSpin plant II miniSilica bindingMacherey-NagelTeaUncu et al. [137]
Plant tissueOsathanunkul et al. [138]
SeedsCosta et al. [126]
Dried plant tissueOsathanunkul et al. [139]
Leaves, pills, cone, stem, roots, barkLlongueras et al. [140]

NuceloSpin Tissue KitsSilica bindingMacherey-NagelTea, roots, capsule, liquid, root pieces, tabletsWallace et al. [46]

BarkPalhares et al. [141]
Capsule, tabletsBaker [142]
Leavesda Costa et al. [121]
Leaves, flower, rootsPalhares et al. [143]
DNeasy Plant mini kitSilica bindingQiagenLeaves, pills, cone, stem, roots, barkLlongueras et al. [140]
PowderCimino [144]
LeavesAlexander [124]
LeavesEnan and Ahmed [145]
Fruit, powderParvathy et al. [146]

DNeasy96 Plant kitSilica bindingQiagenTeaStoeckle et al. [147]

Plant Genomic DNA KitSilica bindingTiangen BiotechDecoctionXin et al. [148]
Dried bulbusXiang et al. [149]
Leaves, cortexZhang et al. [150]
Crude drug, dry leaf, rootZhu et al. [151]
Root, pills, capsule, tabletsLiu et al. [152]
Dry fruit, leavesDian-Yun et al. [153]
Dry leaves, rootsHu et al. [154]
Dry flower budHou et al. [155]

IBI genomic DNA mini kit (plant, GP1)Silica bindingIBILeaves, pills, cone, stem, roots, barkLlongueras et al. [140]