Isolation and Characterization of Isofraxidin 7-O-(6′-O-p-Coumaroyl)-β-glucopyranoside from Artemisia capillaris Thunberg: A Novel, Nontoxic Hyperpigmentation Agent That Is Effective In Vivo

Figure 2

Effect of compound 1 on melanin content in cultured B16F10 melanoma cells. (a) Cell images with or without the indicated treatment were taken using a digital camera (magnification 100x). Scale bar = 20 μm. Untreated B16F10 show characteristic fibroblastic and spherical cell morphology. Isofraxidin and IFG treated B16F10 melanocytes show increased darkening of the cytoplasm compared to control cells after exposure to 25 μM compound 1 for 2 days. (b) Isofraxidin and compound 1 treated B16F10 cell pellets are more pigmented compared to untreated cells. (c) B16F10 cells were treated with compound 1 for 48 h at 37°C showing increased pigmentation in the melanocytes. (d) B16F10 melanocytes were treated with isofraxidin and compound 1 at the indicated concentrations for 2 days. Compound 1 also increased tyrosinase activity. compared to the untreated control. compared to isofraxidin treatment. AZ = azelaic acid.

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