Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization

Figure 1

Chromatographic profile of E. uniflora leaves. Chromatographic conditions: Supelco Analytical C18 column (250 × 4.6 mm, 5 μm); mobile phase water (A)/acetonitrile (B) (0 min 98% A and 2% B, 5 min 95% A and 5% B, 12 min 80% A and 20% B, 15 min 75% A and 25% B, 18 min 60% A and 40% B, 25 min 20% A and 80% B, 28 min 95% A and 5% B, and 30 min 98% A and 2% B); flow rate of 1.0 mL min⁻¹; λ = 280 nm; and injection volume of 10 μL.