Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of<i> Eugenia uniflora</i> L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization

<div>HPLC-UV chromatograms from ellagic acid analytical standard (a) and<i> E. uniflora</i> leaf extract (b) using the validated conditions: Supelco Analytical C18 column (250 × 4.6 mm, 5 <i>μ</i>m); mobile phase water/acetonitrile (85 : 15); flow rate of 1.0 mL min<sup>−1</sup>; <i>λ</i> = 254 nm; and injection volume of 10 <i>μ</i>L. RT = retention time.</div>

Evidence-Based Complementary and Alternative Medicine

fig2

Figure 2

Figure 2: Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of<i> Eugenia uniflora</i> L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization 

Figure 2 | Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization 