Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization

Table 2

Box Behnken (3³) experimental design and concentrations of ellagic acid (EA) for the UAE of E. uniflora leaves.

	(%)	(°C)	(min)	EA(μg mL^−1)
(1)	40	20	45	21.7
(2)	80	20	45	9.2
(3)	40	40	45	14.5
(4)	80	40	45	8.1
(5)	40	30	30	13.1
(6)	80	30	30	8.7
(7)	40	30	60	20.6
(8)	80	30	60	14.5
(9)	60	20	30	8.0
(10)	60	40	30	11.1
(11)	60	20	60	26.3
(12)	60	40	60	17.3
(13)	60	30	45	15.0
(14)	60	30	45	14.6
(15)	60	30	45	16.4

= ethanol; = temperature; = extraction time.