Liensinine and Nuciferine, Bioactive Components of Nelumbo nucifera, Inhibit the Growth of Breast Cancer Cells and Breast Cancer-Associated Bone Loss
Liensinine and nuciferine inhibited the viability, migration, and invasion of breast cancer cells. (a) Chemical structures of liensinine and nuciferine. (b) MDA-MB-231 or MCF-7 cells were treated with various concentrations of liensinine and nuciferine in serum-free media for 24 h. Cell viability was determined by an MTT assay. (c) In the presence of liensinine or nuciferine at the indicated concentrations, MDA-MB-231 or MCF-7 cells were added to transwell chamber and attracted by 5% FBS for 24 h. (d) MDA-MB-231 or MCF-7 cells were seeded into the matrigel-based upper chamber with serum-free media containing liensinine or nuciferine. The lower chamber was filled with 600 μl of media containing 5% FBS and liensinine or nuciferine for 24 h. Data are expressed as the mean ± SE. and versus untreated cells.