Antioxidant and Anti-Inflammatory Effects of Herbal Formula SC-E3 in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

Figure 7

Effects of SC-E3 on the Nrf2/HO-1 signaling pathway in RAW 264.7 macrophages. (a) Induction of HO-1 by SC-E3. Cells were treated with different concentrations of SC-E3 (50, 100, 300, or 500 μg/mL) for 18 h. (b) Cells were treated with 300 μg/mL SC-E3 for the indicated times. (c) Nuclear accumulation of Nrf2 by SC-E3. Nrf2 was immunoblotted in the nuclear fractions of cells treated with 300 μg/mL of SC-E3 for the indicated times. (d) Immunofluorescence images of the nuclear translocation of Nrf2 induced by SC-E3. RAW 264.7 cells were treated with 300 μg/mL of SC-E3 for 3 h. (e) Blocking of the inhibitory effect of SC-E3 on LPS-induced NO production by SnPP (an HO-1 inhibitor). RAW 264.7 cells were pretreated with SC-E3 (300 μg/mL) for 1 h in the presence or absence of SnPP (50 nM, 30 min) and then stimulated with LPS (1 μg/mL) for 18 h. .

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