Rutin Protects against Pirarubicin-Induced Cardiotoxicity through TGF-β1-p38 MAPK Signaling Pathway

Figure 6

Western blot analysis of protein expression. (a) Representative data of Western blot analysis. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as internal control. Semiquantitative analysis of TGF-β1, p-p38 MAPK, cleaved Caspase-9, Caspase-7, and Caspase-3 levels were presented in (b–f). The relative expression of p-p38 MAPK was normalized to that of total p38 MAPK and others were normalized to that of GAPDH. versus control; versus THP; versus DZR + THP. 1 = control, 2 = THP group, 3 = DZR + THP group, 4 = RUT + THP group, 5 = SB203580 + THP group, and 6 = SB203580 + THP + RUT group. DZR, dexrazoxane; MAPK, mitogen-activated protein kinase; RUT, rutinum; THP, pirarubicin; TGF-β1, transforming growth factor-β1.

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