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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 1767301, 11 pages
https://doi.org/10.1155/2017/1767301
Research Article

Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis

1Key Laboratory of Acupuncture and Immunological Effects, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China
2Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China
3Yueyang Clinical Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
4Department of Acupuncture and Moxibustion, Shanghai Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 200437, China

Correspondence should be addressed to Jing Li and Huan-gan Wu

Received 4 September 2016; Accepted 27 December 2016; Published 26 January 2017

Academic Editor: Ke Ren

Copyright © 2017 Yan Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. This study explored the mechanism of herb-partitioned moxibustion (HM) on dextran sulfate sodium- (DSS-) induced ulcerative colitis (UC) from the miRNA perspective. Methods. Rats were randomly divided into 3 groups [normal control (NC) group, UC model (UC) group, and herb-partitioned moxibustion (UCHM) group]. The UC and UCHM groups were administered 4% DSS for 7 days. The UCHM group received HM at the Tianshu (bilateral, ST25). The effect of HM on UC was observed and the miRNA expression profile in the colon tissues was analyzed. Results. Compared with the UC group, the body weights were significantly higher in the UCHM group on day 14 (); the macroscopic colon injury scores and microscopic histopathology scores in the UCHM group decreased (); and there were 15 differentially expressed miRNAs in the UCHM group. The changes in miR-184 and miR-490-5p expression levels on the UC were reversed by HM intervention. Validation using qRT-PCR showed that two miRNAs expression trend was consistent with the sequencing results. Conclusion. HM at ST25 might regulate miR-184 and miR-490-5p expression, act on the transcription of their target genes to regulate inflammatory signaling pathways, and attenuate inflammation and tissue injury in the colons of rats with DSS-induced UC.