Evidence-Based Complementary and Alternative Medicine / 2017 / Article / Fig 1

Research Article

Yiqi Huayu Jiedu Decoction Inhibits the Invasion and Metastasis of Gastric Cancer Cells through TGF-β/Smad Pathway

Figure 1

YHJD inhibited gastric cancer MGC-803 cells’ proliferation, adhesion, migration, and invasion. (a) TGF-β (10 ng/mL), TGF-β/Smad (10 μM/L SB431542), and YHJD (2 mg/mL) were administered to cells and cultivated for 24 h, 48 h, and 72 h, and the inhibition rate (%) was determined using MTT assays and was expressed as (1 − absorbance of test sample/absorbance of control) × 100%. (b) YHJD inhibited proliferation of gastric cancer cells and affected cells’ morphology. (c) Cell adhesion assay was used to detect MGC-803 cells’ adhesion for GC cells. The bar chart was the inhibition rate of cell adhesion of each group at different periods (30, 60, 90, and 120 min). (d) Wound assay detected migration of different groups for MGC-803 cells, which were photographed 24 h after wounding (100x) to record their migration. (e) The bar chart shows wound-healing percentage of gastric cancer MGC-803 cells 24 h after being wounded. (f) Transwell assay detected the effects of each group’s invasion effect on MGC-803 cells. Light microscopic images of different concentrations (400x) were taken. (g) The bar chart shows the number of MGC-803 cells that migrated across 8 μm diameter pores to the lower chamber. Data are expressed as the mean ± SD of three experiments ( compared with control group, compared with control group).