Yiqi Huayu Jiedu Decoction Inhibits the Invasion and Metastasis of Gastric Cancer Cells through TGF-β/Smad Pathway
The levels of EMT and TGF-β/Smad pathway related proteins in the transplanted tumor were determined by immunohistochemical assay. (a) E-cadherin. (b) N-cadherin. (c) Vimentin. (d) TGF-βR I. (e) p-Smad2. (f) p-Smad3. (g) Snail. (h) Slug. Protein of (I) (control group), (II) (5-FU group), (III) (high-dose group), and (IV) (low-dose group) turned brown granular in tumor staining. Positive staining of E-cadherin, N-cadherin, and vimentin focused in the cytoplasm, a few in the envelope, brownish yellow granular. TGF-βR I was mainly stained in the cell membrane. p-Smad2 and p-Smad3 in the cytoplasm, a few in the envelope. Snail in the cytoplasm, a few in the nucleus. Slug in the nucleus. (i) The bar chart indicates each molecule’s mean density. (j) The bar chart indicates expression positive rate of Slug in metastatic tumor tissue sections. Data are expressed as the mean ± SD of three experiments ( compared with control group, compared with control group).