Evaluation of an Aqueous Extract from Horseradish Root (Armoracia rusticana Radix) against Lipopolysaccharide-Induced Cellular Inflammation Reaction

Figure 2

Effect of horseradish root on 5-LOX pathway activation. (a) PBMC were stimulated with 1 μg/mL LPS for 6 h after pretreatment with the aqueous extract for 3 h. Total cell lysate was analyzed using immunoblotting. The picture shows representative blots of 5-LOX and β-actin (loading control). (b) 3 h treatment of PBMC with the plant extract was followed by 15 min incubation with 1 μg/mL LPS and subsequently with 1 μM fMLP for the indicated times. LTB4 release was photometrically quantified in the supernatants and calculated relative to the control; bars are mean ± SEM (). ; .

(a)

(b)