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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 1960584, 13 pages
Research Article

Involvement of Normalized Glial Fibrillary Acidic Protein Expression in the Hippocampi in Antidepressant-Like Effects of Xiaoyaosan on Chronically Stressed Mice

1School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China
2Henan University of Chinese Medicine, Henan, China
3School of Pre-Clinical Medicine, Hubei University of Chinese Medicine, Wuhan, China
4Beijing Chaoyang Hospital, Capital Medical University, Beijing, China
5School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China

Correspondence should be addressed to Jia-Xu Chen and Zhi-Ping Lv

Received 15 August 2016; Revised 29 November 2016; Accepted 26 December 2016; Published 2 March 2017

Academic Editor: Junqing Yang

Copyright © 2017 Xiu-Fang Ding et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The research has only yielded a partial comprehension of MDD and the mechanisms underlying the antidepressant-like effects of XYS. Therefore, in this study, we aimed to explore the effects of XYS on chronic unpredictable mild stress- (CUMS-) induced changes in the neuronal and the astrocytic markers in the mouse hippocampus. The physical states and depressive-like behaviors in mice with CUMS were recorded. The serum contents of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) were measured. The protein and mRNA expressions and the immunoreactivities of glial fibrillary acidic protein (GFAP) and neuronal nuclei (NeuN) in mouse hippocampus were detected using a Western blot, qRT-PCR, and immunohistochemical staining, respectively. XYS treatment markedly improved the physical state and depressive-like behaviors in mice subjected to CUMS compared with the model group, and the serum contents of BDNF and GDNF were significantly upregulated. XYS treatment also elevated the protein and mRNA levels, as well as the immunoreactivity of GFAP in the hippocampus. However, CUMS did not influence NeuN expression. In conclusion, these results reveal that chronic administration of XYS elicits antidepressant-like effects in a mouse model of depression and may normalize glial fibrillary acidic protein expression in the hippocampi of mice with CUMS.