Influences of Realgar-Indigo naturalis, A Traditional Chinese Medicine Formula, on the Main CYP450 Activities in Rats Using a Cocktail Method

Table 2

Intra-/interday accuracy and precision of the developed assay ().

Number	QC conc. (μg⋅ml^−1)	Intraday			Interday
		Calc. conc. (μg⋅ml^−1)	RSD (%)	Accuracy (%)	Calc. conc. (μg⋅ml^−1)	RSD (%)	Accuracy (%)
Caffeine	5.38	5.32 ± 0.01	0.14	98.97 ± 0.14	5.32 ± 0.04	0.79	98.81 ± 0.78
	10.75	9.89 ± 0.03	0.30	92.02 ± 0.27	9.93 ± 0.07	0.66	92.38 ± 0.61
	51.80	47.76 ± 0.48	1.00	92.20 ± 0.92	46.91 ± 1.74	3.70	90.56 ± 3.35
Chlorzoxazone	10.16	10.63 ± 0.03	0.25	104.60 ± 0.26	10.62 ± 0.07	0.64	104.48 ± 0.67
	20.32	19.96 ± 0.03	0.17	98.21 ± 0.17	20.29 ± 0.43	2.11	99.85 ± 2.10
	101.60	96.35 ± 0.40	0.42	94.84 ± 0.40	96.72 ± 0.56	0.57	95.20 ± 0.55
Tolbutamide	11.13	10.71 ± 0.06	0.56	96.22 ± 0.54	10.59 ± 0.07	0.63	95.17 ± 0.60
	22.26	20.08 ± 0.05	0.23	90.22 ± 0.21	19.97 ± 0.25	1.23	89.69 ± 1.10
	109.60	96.34 ± 0.38	0.39	87.90 ± 0.34	96.05 ± 1.70	1.77	87.64 ± 1.55
Midazolam	11.01	10.64 ± 0.02	0.18	96.60 ± 0.18	10.58 ± 0.17	1.56	96.12 ± 1.50
	22.02	19.88 ± 0.04	0.20	90.28 ± 0.18	19.65 ± 0.18	0.92	89.24 ± 0.82
	105.30	96.37 ± 0.38	0.40	91.52 ± 037	94.66 ± 1.93	2.04	89.90 ± 1.83