Influences of Realgar-Indigo naturalis, A Traditional Chinese Medicine Formula, on the Main CYP450 Activities in Rats Using a Cocktail Method

Table 4

Stability of analytes in rat plasma ().

Number	QC conc. (μg⋅ml^−1)	Room temperature stability			Freeze-thaw stability
		Calc. conc. (μg⋅ml^−1)	RSD (%)	Accuracy (%)	Calc. conc. (μg⋅ml^−1)	RSD (%)	Accuracy (%)
Caffeine	5.38	5.30 ± 0.05	0.90	98.54 ± 0.88	5.27 ± 0.08	1.44	97.95 ± 1.41
	10.75	9.74 ± 0.14	1.44	90.92 ± 1.3	9.87 ± 0.12	1.22	91.66 ± 1.09
	51.80	47.38 ± 0.89	1.88	91.30 ± 1.59	47.82 ± 1.07	2.23	92.24 ± 1.85
Chlorzoxazone	10.16	10.63 ± 0.09	0.86	104.66 ± 0.90	10.59 ± 0.08	0.79	104.28 ± 0.83
	20.32	20.20 ± 0.35	1.74	99.98 ± 2.05	20.40 ± 0.43	2.13	100.02 ± 2.10
	101.60	96.23 ± 0.68	0.70	94.67 ± 0.61	96.41 ± 0.48	0.49	94.81 ± 0.46
Tolbutamide	11.13	10.64 ± 0.17	1.62	95.57 ± 1.55	10.47 ± 0.29	2.74	94.06 ± 2.57
	22.26	20.32 ± 0.41	2.00	91.06 ± 1.71	20.03 ± 0.25	1.25	89.94 ± 1.01
	109.60	95.52 ± 2.17	2.27	87.27 ± 1.79	94.39 ± 1.29	1.37	86.30 ± 1.14
Midazolam	11.01	10.70 ± 0.29	2.71	97.21 ± 2.63	10.51 ± 0.13	1.22	95.45 ± 1.17
	22.02	19.90 ± 0.05	0.27	90.40 ± 024	19.78 ± 0.11	0.55	89.76 ± 0.49
	105.30	95.78 ± 0.55	0.57	91.12 ± 0.61	95.20 ± 2.52	2.65	90.28 ± 2.16