Table 4: Stability of analytes in rat plasma ().

NumberQC conc. (μg⋅ml−1)Room temperature stabilityFreeze-thaw stability
Calc. conc. (μg⋅ml−1)RSD (%)Accuracy (%)Calc. conc. (μg⋅ml−1)RSD (%)Accuracy (%)

Caffeine5.385.30 ± 0.050.9098.54 ± 0.885.27 ± 0.081.4497.95 ± 1.41
10.759.74 ± 0.141.4490.92 ± 1.39.87 ± 0.121.2291.66 ± 1.09
51.8047.38 ± 0.891.8891.30 ± 1.5947.82 ± 1.072.2392.24 ± 1.85

Chlorzoxazone10.1610.63 ± 0.090.86104.66 ± 0.9010.59 ± 0.080.79104.28 ± 0.83
20.3220.20 ± 0.351.7499.98 ± 2.0520.40 ± 0.432.13100.02 ± 2.10
101.6096.23 ± 0.680.7094.67 ± 0.6196.41 ± 0.480.4994.81 ± 0.46

Tolbutamide11.1310.64 ± 0.171.6295.57 ± 1.5510.47 ± 0.292.7494.06 ± 2.57
22.2620.32 ± 0.412.0091.06 ± 1.7120.03 ± 0.251.2589.94 ± 1.01
109.6095.52 ± 2.172.2787.27 ± 1.7994.39 ± 1.291.3786.30 ± 1.14

Midazolam11.0110.70 ± 0.292.7197.21 ± 2.6310.51 ± 0.131.2295.45 ± 1.17
22.0219.90 ± 0.050.2790.40 ± 02419.78 ± 0.110.5589.76 ± 0.49
105.3095.78 ± 0.550.5791.12 ± 0.6195.20 ± 2.522.6590.28 ± 2.16