Evaluation of Anti-Inflammatory Activities of Qingre-Qushi Recipe (QRQS) against Atopic Dermatitis: Potential Mechanism of Inhibition of IL-33/ST2 Signal Transduction

Figure 8

(a) Effect of QRQS on TNF-– or TNF- +IFN--induced HaCaT cells to produce IL-33 mRNA and protein. TNF- (50 ng/mL) or TNF- (50 ng/mL) + IFN- (50 ng/mL) stimulated HaCaT cells were cultured with different concentrations of QRQS solution for 24 h. RT-PCR was used to detect the IL-33 mRNA secretion. (b) The influence of different time points in different concentration decoctions on the expression of the IL-33 protein. The TNF- (50 ng/mL) + IFN- (50 ng/mL) combined with HaCaT cells were treated with different concentrations (0.125 g/mL, 0.5 g/mL, and 2.0 g/mL) of QRQS, and, at different time points (24, 32, 48, and 56 h), supernatants were collected. The IL-33 protein expression was detected by ELISA. The data are shown as mean ± SEMs, , compared with the blank group; , , and , compared with the model group.

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