Evaluation of Anti-Inflammatory Activities of Qingre-Qushi Recipe (QRQS) against Atopic Dermatitis: Potential Mechanism of Inhibition of IL-33/ST2 Signal Transduction

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Cytokine</td><td class="align_center">Forward primer (5′- 3′)</td><td class="align_center">Reverse primer (5′- 3′)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">IL-33</td><td class="align_center">ATTTCCCCGGCAAAGTTCAG</td><td class="align_center">AACGGAGTCTCATGCAGTAGA</td></tr><tr><td class="align_left">ST2</td><td class="align_center">AGAAGCCCCAACTTGAATAAGAC</td><td class="align_center">TCTGATCCACGTACTGTCGAG</td></tr><tr><td class="align_left">TNF-<svg height="6.1673pt" id="M18" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -5.96091 7.51131 6.1673" width="7.51131pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M545 106L524 126C493 85 467 65 455 65C438 65 427 113 405 238C448 295 498 362 543 439L533 448L478 435C453 386 423 331 398 295H395C370 404 347 448 282 448C169 448 23 309 23 153C23 54 65 -12 128 -12C203 -12 283 70 339 155H341C360 29 380 -12 411 -12C444 -12 491 11 545 106ZM333 204C265 95 210 54 169 54C137 54 113 96 113 171C113 302 191 405 252 405C301 405 318 306 333 204Z" id="g113-223"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="568" vert-adv-y="568" xpluscharwidth="7.384"></glyph.data></g></svg></td><td class="align_center">CTGAACTTCGGGGTGATCGG</td><td class="align_center">GGCTTGTCACTCGAATTTTGAGA</td></tr><tr><td class="align_left">IFN-<svg height="9.39034pt" id="M19" style="vertical-align:-3.42943pt" version="1.1" viewbox="-0.0498162 -5.96091 6.63704 9.39034" width="6.63704pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M478 372C478 418 458 448 431 448C409 448 389 431 389 410C389 404 391 400 394 395C398 388 406 371 406 348C406 253 308 122 251 51H249C254 122 249 257 231 336C212 421 189 448 159 448C126 448 75 412 23 327L48 306C83 354 103 371 115 371C125 371 134 360 144 334C185 224 192 64 183 -19C146 -100 116 -202 110 -244L125 -261C154 -259 208 -234 222 -220C222 -194 225 -84 235 -23C247 -3 273 36 308 79C379 165 478 288 478 372Z" id="g113-225"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="501" vert-adv-y="501" xpluscharwidth="6.513"></glyph.data></g></svg></td><td class="align_center">ACAGCAAGGCGAAAAGGATG</td><td class="align_center">TGGTGGACCACTCGGATGA</td></tr><tr><td class="align_left">IL-4</td><td class="align_center">GGTCTCAACCCCCAGCTAGT</td><td class="align_center">GCCGATGATCTCTCTCAAGTGAT</td></tr><tr><td class="align_left">IL-13</td><td class="align_center">CCTGGCTCTTGCTTGCCTT</td><td class="align_center">GGTCTTGTGTGATGTTGCTCA</td></tr><tr><td class="align_left">GAPDH</td><td class="align_center">AGGTCGGTGTGAACGGATTTG</td><td class="align_center">GGGGTCGTTGATGGCAACA</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Sequences of the oligonucleotides used in RT-PCR reactions.

Evidence-Based Complementary and Alternative Medicine

tab1

Table 1

Table 1: Evaluation of Anti-Inflammatory Activities of Qingre-Qushi Recipe (QRQS) against Atopic Dermatitis: Potential Mechanism of Inhibition of IL-33/ST2 Signal Transduction 

Table 1 | Evaluation of Anti-Inflammatory Activities of Qingre-Qushi Recipe (QRQS) against Atopic Dermatitis: Potential Mechanism of Inhibition of IL-33/ST2 Signal Transduction 