Icariin Prevents H2O2-Induced Apoptosis via the PI3K/Akt Pathway in Rat Nucleus Pulposus Intervertebral Disc Cells
Icariin protects nucleus pulposus (NP) cells from H2O2-induced apoptosis. Bcl-2, Bax, and caspase-3 were detected by Western blotting. Bcl-2 levels are descended in NP cells treated with H2O2 ( versus control group) and Bcl-2 levels are increased in NP cells treated with H2O2 and icariin when compared with the H2O2 alone group ( versus H2O2 group) and decreased when the PI3K/Akt pathway is blocked with LY294002 ( versus group (C)). Caspase-3 and Bax are decreased in icariin-treated cells ( versus control group). The protective effect of icariin could be impaired by the presence of LY294002 to block the PI3K/Akt pathway ( versus H2O2 group, versus group (C)). In addition, LY294002 alone exacerbates H2O2-induced damage (, versus H2O2 group).
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