Research Article

Rapid Screening of Potential Phosphodiesterase Inhibitors from the Roots of Ilex pubescens Hook. et Arn. Using a Combination of Ultrafiltration and LC–MS

Table 2

PDE inhibitory activity (IC50) and binding effect with PDEI expressed in LC signal enhancement factor of major components of I. pubescens roots.

Compound Inhibitory activity (IC50, μM) Enhancement factor with PDEI Contents (mg/g
PDEIPDE5A10 U/mL20 U/mL

Isochlorogenic acid B (3)779.5193.50.23
Isochlorogenic acid C (5)0.27
Ilexsaponin B2 (8)477.548.81.24
Ilexsaponin A1(9)332.022.42.53
Ilexsaponin B1 (10)853.71801.72.18
Ilexgenin A (11)837.7176.68.96

factor (%) = (amount of compound specifically bound)/(total amount of compound in incubation) × 100. of individual compounds expressed as mg/g of dry plant powder.