Anticancer Effect of Nemopilema nomurai Jellyfish Venom on HepG2 Cells and a Tumor Xenograft Animal Model

Figure 4

Effects of NnV on HepG2 tumor growth and induction of apoptosis. (a) Mice were orthotopically implanted with HepG2 cells and treated with NnV (0.1 and 0.3 mg/kg, I.P) for 20 day as described in Materials and Methods. Tumor sizes were measured by microcalipers every two days and average tumor volumes were representing the total tumor volumes per mouse. (b) On day 22, mice were sacrificed and the tumors were collected. Representative photographs of tumors are shown. (c) Induction of apoptosis was analyzed by TUNEL assay. As shown in the right panel, number of TUNEL-positive cells presented as folds compared with control group. (d) Tumor tissues were analyzed by immunohistochemistry to identify Ki-67 and p-Akt at Ser473 and Thr308 and E-cadherin expressions (×200 objective magnification). The results are expressed as the mean ± SD. was considered to indicate statistical significance compared with control group.

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