Anticancer Effect of Nemopilema nomurai Jellyfish Venom on HepG2 Cells and a Tumor Xenograft Animal Model

Figure 5

Isolation of anticancer component from NnV. (a) NnV preincubated with tetracycline (Tetra) 150 μM for 3 h was treated in HepG2 cells for 24 h and then MTT assay was performed. (b) Crude venom was dissolved in 10 mM Tris-HCl (pH 7.8) buffer and centrifuged for 30 min at 13,000 ×g. The supernatant was introduced in a DEAE column and the proteins were eluted with a NaCl linear gradient of 0 to 80% at flow rate of 1 ml/min. (c) All fractions were evaluated for cytotoxicity in HepG2 cells.

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