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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 2752716, 12 pages
https://doi.org/10.1155/2017/2752716
Research Article

Anticancer Effect of Nemopilema nomurai Jellyfish Venom on HepG2 Cells and a Tumor Xenograft Animal Model

1Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea
2College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea
3Department of Anatomy, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea
4Headquarters for Marine Environment, National Fisheries Research & Development Institute, Shiran-ri, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea

Correspondence should be addressed to Euikyung Kim; rk.ca.ung@mike

Received 2 February 2017; Accepted 5 June 2017; Published 13 July 2017

Academic Editor: Youn C. Kim

Copyright © 2017 Hyunkyoung Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: NnV induces cytotoxicities of several normal cell lines; WB-F344 (rat liver epithelial cell), HaCaT (human keratinocyte), HDF (Human dermal fibroblast) and H9C2 (rat cardiomyocyte). Each cells were treated with various concentrations of NnV for 24 h, and MTT assay was evaluated. The data shown are the mean ± SD of six independent experiments. *p<0.05 was considered to indicate statistical significance compared with non-treated controls.

Supplementary Figure 2: Toxicity evaluation of NnV in liver and heart tissues. To ensure the safety of NnV treatment, mice bearing HepG2 cells were sacrificed at the end of the experiment, and blood, liver and heart tissues were collected by routine procedure. (A) The parameters of liver and heart functions in control and NnV-treated mice were analyzed using Chemistry. (B) Body weights in the mice of control and NnV-treated group were checked during experiments. (C) Liver and heart tissues were evaluated by hematoxylin and eosin (H&E) staining (× 200 objective magnification). There are no histological change in NnV-treated groups.

  1. Supplementary Material