DJC Suppresses Advanced Glycation End Products-Induced JAK-STAT Signaling and ROS in Mesangial Cells

Figure 5

DJC inhibited AGEs-induced ROS and NADPH oxidase activity. Cells were treated with 5% FBS (control), AGEs, and DJC (0.125, 0.5, and 2 mg/ml) or APO (20 M) in the presence of AGEs for 48 h. The cellular ROS was measured by DCFH-DA (a), and the NADPH oxidase activity was assayed by lucigenin-enhanced chemiluminescence (b). (c) Effects of DJC on cell viability. GMCs were treated with various concentrations of DJC for 48 h. Cell viability was determined by MTT assay. Control in the absence of DJC and AGEs was taken as 100%. The results were expressed as mean ± SD of three independent experiments. The columns and error bars represent the mean and SD ( per group). P < 0.05, P < 0.01, and P < 0.001 versus Ctrl; P < 0.05 and P < 0.01 versus AGEs.

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