Effect of Huayu Tongluo Herbs on Reduction of Proteinuria via Inhibition of Wnt/β-Catenin Signaling Pathway in Diabetic Rats

Table 2

Primers for rat genes used in real-time PCR.

Gene	Forward primer (5′→3′)	Reverse primers (5′→3′)	Length of product (bp)
β-Actin	ACCCGCGAGTACAACCTTCT	TTCAGGGTCAGGATGCCTCT	266 bp
Wnt4	AGCCCACAGGGTTTCCA	GCTCGCCAGCATGTCTTT	233 bp
GSK-3β	GTCCGATTGCGGTATT	GTGTCTGGCGACTCTGTA	108 bp
β-Catenin	AACGGCTTTCGGTTGAGCTG	TGGCGATATCCAAGGGCTTC	147 bp
E-Cadherin	TGCTCCTACTGTTTCTACG	CTTCTCCACCTCCCTCTT	111 bp
ILK	AATGGGACCCTGAACAAA	GAGCCTGGCAAGCACCTA	244 bp
TGF-β1	ATGGTGGACCGCAACAAC	TGAGCACTGAAGCGAAAGC	329 bp