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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 3476407, 10 pages
Research Article

The Changes in Rats with Sciatic Nerve Crush Injury Supplemented with Evening Primrose Oil: Behavioural, Morphologic, and Morphometric Analysis

1Department of Anatomy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
2Department of Pharmaceutical Sciences, Faculty of Pharmacy, Cyberjaya University College of Medical Sciences (CUCMS), Cyberjaya, Selangor, Malaysia

Correspondence should be addressed to Junedah Sanusi;

Received 27 November 2016; Revised 17 March 2017; Accepted 10 April 2017; Published 23 May 2017

Academic Editor: Jintanaporn Wattanathorn

Copyright © 2017 Danial Ramli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Nerve crush injuries are commonly used models for axonotmesis to examine peripheral nerve regeneration. As evening primrose oil (EPO) is rich in omega-6 essential fatty acid component and gamma-linolenic acid, studies have shown the potential role of EPO in myelination. Seventy-two healthy adult Sprague-Dawley rats were classified into three groups: normal group, control group, and experimental group. The result indicates that there was significant difference in toe-spreading reflex between the normal and the control groups (, ) and the normal and the EPO groups (, ) and significant difference between EPO and the control groups (, ). Regeneration of axons and myelin in nerve fibre in the EPO-treated group developed better and faster than in the control group. In the control group, the shape of the axon was irregular with a thinner myelin sheath. In the experimental group, the shape of the axons, the thickness of the myelin sheath, and the diameter of the axons were almost the same as in the normal group. In conclusion, EPO supplementation may be beneficial as a therapeutic option for disturbances of nerve interaction.