Research Article
Thunbergia laurifolia Exhibits Antifibrotic Effects in Human Hepatic Stellate Cells
Figure 4
Effects of TL extract on MMP-9 activity and mRNA expression of TIMP-1, MMP-2, and MMP-9 genes. MMP-9 activity of LX-2 cells were investigated by gelatin zymography. Bar graphs in zymogram represent the percentage of MMP-9 activity (a). indicates statistical significance from no TGF- group. indicates statistical significance from TGF--treated group. Gene expression of TIMP-1, MMP-2, and MMP-9 was measured by quantitative RT-PCR (b). GAPDH was used as an internal control. indicates statistical significance from TGF--treated group.
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