Tetramethylpyrazine Protects against Early Brain Injury after Experimental Subarachnoid Hemorrhage by Affecting Mitochondrial-Dependent Caspase-3 Apoptotic Pathway

Figure 4

Expressions of cyt c and Smac, cleaved caspase-3, and bcl-2. (a) for Western blotting images; (b) for cyt c in different groups; (c) for Smac; (d) for cleaved caspase-3; (e) for bcl-2. The bar graph shows the ratio of the cyt c, Smac, cleaved caspase-3, and bcl-2 integrated density value (IDV) to GAPDH IDV for each experimental condition. Data represent the mean ± SD of 3 groups ( in each group). As figures showed, the increase of cytoplasmic cyt c and Smac took place after SAH and it was significantly inhibited by TMP treatment. Sham for sham-operation group; SAH for subarachnoid hemorrhage; TMP for tetramethylpyrazine; GAPDH for glyceraldehyde 3-phosphate dehydrogenase; cyt c for cytochrome c; Smac for second mitochondria-derived activator of caspases; bcl-2 for B-cell lymphoma 2. ( compared with the sham-operation group, compared with the SAH + Vehicle group.)

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