Tetramethylpyrazine Protects against Early Brain Injury after Experimental Subarachnoid Hemorrhage by Affecting Mitochondrial-Dependent Caspase-3 Apoptotic Pathway

Figure 5

TUNEL-positive and apoptosis index. (a–c) Representative microphotographs of DAPI-positive cells (blue dots); (d–f) representative microphotographs of TUNEL-positive cells (green dots); (g–i) representative microphotographs merging DAPI-positive cells and TUNEL-positive cells. (j) Apoptosis index (AI) was defined as the mean percentage of TUNEL-positive cells out of all DAPI-positive cells in six cortical microscopic fields (×400 magnification). As figures showed, the apoptosis triggered by SAH was improved by TMP. Bars represent the mean ± SD ( in each group) ( compared with the sham-operation group, compared with the SAH+ Vehicle group.) Scale bar indicates 20 μm.

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